Increased vascular permeability contributes to many diseases, including acute respiratory distress syndrome, cancer and inflammation. Most past work on vascular barrier function has focused on soluble regulators, such as tumour-necrosis factor-α. Here we show that lung vascular permeability is controlled mechanically by changes in extracellular matrix structure. Our studies reveal that pulmonary vascular leakage can be increased by altering extracellular matrix compliance in vitro and by manipulating lysyl oxidase-mediated collagen crosslinking in vivo. Either decreasing or increasing extracellular matrix stiffness relative to normal levels disrupts junctional integrity and increases vascular leakage. Importantly, endotoxin-induced increases of vascular permeability are accompanied by concomitant increases in extracellular matrix rigidity and lysyl oxidase activity, which can be prevented by inhibiting lysyl oxidase activity. The identification of lysyl oxidase and the extracellular matrix as critical regulators of lung vascular leakage might lead to the development of new therapeutic approaches for the treatment of pulmonary oedema and other diseases caused by abnormal vascular permeability.

血管通透性增加導(dǎo)致許多疾病，包括急性呼吸窘迫綜合征，癌癥和炎癥。過去有關(guān)血管屏障功能的大多數(shù)工作都集中在可溶性調(diào)節(jié)劑上，例如腫瘤壞死因子-α。在這里，我們顯示肺血管通透性是由細(xì)胞外基質(zhì)結(jié)構(gòu)的變化機械地控制的。我們的研究表明，通過改變體外細(xì)胞外基質(zhì)的順應(yīng)性和在體內(nèi)操縱賴氨酰氧化酶介導(dǎo)的膠原蛋白交聯(lián)可以增加肺血管滲漏。相對于正常水平降低或增加細(xì)胞外基質(zhì)硬度都會破壞連接完整性并增加血管滲漏。重要的，內(nèi)毒素誘導(dǎo)的血管通透性增加伴隨著細(xì)胞外基質(zhì)剛性和賴氨酰氧化酶活性的同時增加，這可以通過抑制賴氨酰氧化酶活性來預(yù)防。賴氨酰氧化酶和細(xì)胞外基質(zhì)作為肺血管滲漏的關(guān)鍵調(diào)節(jié)劑的鑒定可能會導(dǎo)致開發(fā)新的治療方法，用于治療由異常的血管通透性引起的肺水腫和其他疾病。

biomomentum多軸機械測試儀Mach-1
 Mach-1多軸機械測試儀是模塊化集成壓縮，拉伸，剪切，摩擦，扭轉(zhuǎn)和3D壓痕映射、電位分布等測試設(shè)備

   biomomentum至1999年以來，專注用于測試生物材料，組織和關(guān)節(jié)軟骨機-電特性產(chǎn)品的設(shè)計、開發(fā)、制造和商業(yè)化的創(chuàng)新解決方案20余年。
    其mach-1多軸向多功能組織材料機械特性測試分析系統(tǒng)已經(jīng)成為組織材料機械-電位測試分析的黃金標(biāo)準(zhǔn)。

  1、多功能、多軸向，適用樣品范圍廣：

•1.1、從骨等硬組織材料到腦組織、眼角膜等極軟的組織材料

•1.2、從粗的椎間盤的樣品到極細(xì)的單纖維絲

2、力學(xué)類型測試分析功能齊全：

2.1、模塊化集成壓縮、張力、剪切、摩擦、扭轉(zhuǎn)、穿刺、摩擦和非平面壓痕、3D厚度、3D表面輪廓等各種力學(xué)類型支持,微觀結(jié)構(gòu)表征及動態(tài)力學(xué)分析研究

2.2、多物理場耦合加載測試

•3、通高量壓痕、壓縮測試分析（48孔板中壓痕測試分析）

•4、高精度、高分辨率:

•4.1、位移分辨率達0.1um

•4.2、力分辨率達0.025mN

•4.3、樣品直徑小25um

•5、行程范圍廣：50-250mm

•6、體積小巧、可放入培養(yǎng)箱內(nèi)

•7 、DIC （Digital Image Correlation)數(shù)字圖像相關(guān)法非接觸式的高精度位移、應(yīng)變測量

•9、活性組織電位分布測試分析

•10、產(chǎn)品成熟，文獻量達上千篇